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目的:研究小檗胺对人肝癌SMMC-7721细胞增殖作用的影响及可能的机制。方法:经不同浓度小檗胺处理体外培养的肝癌SMMC-7721细胞,采用MTT法检测细胞增殖的变化;采用PI染色法检测肿瘤细胞周期分布的变化;采用RT-PCR和Western blot法检测肿瘤细胞中Cyclin B1、Cyclin D1、p21和p27的表达水平。结果:与溶剂对照组比较,不同浓度的小檗胺均可明显抑制人肝癌SMMC-7721细胞增殖。PI染色结果显示,小檗胺可明显地诱导肝癌细胞周期阻滞于G0/G1期(P<0.05)。RT-PCR和Western blot结果均显示,应用小檗胺处理肿瘤细胞后,p21和p27的表达明显升高,而Cyclin B1和Cyclin D1的表达水平明显下降(P<0.05)。结论:小檗胺能抑制人肝癌SMMC-7721细胞增殖,并使其细胞周期阻滞于G0/G1期,其机制可能与其上调p21和p27的表达和下调Cyclin B1和Cyclin D1的表达有关。
Objective: To study the effect of berbamine on the proliferation of human hepatoma SMMC-7721 cells and its possible mechanism. Methods: The hepatocellular carcinoma SMMC-7721 cells were treated with different concentrations of berbamine. The cell proliferation was detected by MTT assay. The cell cycle distribution was detected by PI staining. The expression of tumor cells was detected by RT-PCR and Western blot Cyclin B1, Cyclin D1, p21 and p27 expression levels. Results: Compared with the solvent control group, different concentrations of berbamine could significantly inhibit the proliferation of human hepatoma SMMC-7721 cells. PI staining showed that berbamine could obviously induce cell cycle arrest in G0 / G1 phase (P <0.05). The results of RT-PCR and Western blot showed that the expression of p21 and p27 was significantly increased, while the expression of Cyclin B1 and Cyclin D1 was significantly decreased (P <0.05) after treatment with berbamine. CONCLUSION: Berbamine inhibits the proliferation of human hepatocellular carcinoma SMMC-7721 cells and arrests the cell cycle in G0 / G1 phase. Its mechanism may be related to its up-regulation of p21 and p27 expression and down-regulation of the expression of Cyclin B1 and Cyclin D1.