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目的探讨过敏性紫癜患者抗血小板抗体、血小板膜糖蛋白表达量的变化及其临床意义。方法以单克隆抗体为探针,采用流式细胞仪检测45例过敏性紫癜患者血小板α颗粒膜糖蛋白(CD62P)、溶酶体颗粒膜糖蛋白(CD63)、血小板膜表面糖蛋白(CD41)的表达水平；同时采用ELISA法检测抗血小板抗体水平。结果过敏性紫癜患者急性期CD62P、CD63阳性百分数及抗血小板抗体表达水平明显高于缓解期和对照组,差异有统计学意义,CD41表达水平无明显变化。紫癜肾炎型CD62P、CD63及CD41和抗血小板抗体表达水平与混合型比较,差异无统计学意义。紫癜肾炎型及混合型与单纯皮肤型比较,CD62P高于后者,差异有统计学意义；CD63、抗血小板抗体差异无统计学意义。结论过敏性紫癜患者存在体液免疫异常。血小板活化与过敏性紫癜病程变化关系密切。
Objective To investigate the changes of antiplatelet antibody and platelet membrane glycoprotein in patients with allergic purpura and its clinical significance. Methods Monoclonal antibody was used as a probe to detect platelet α - granule membrane glycoprotein (CD62P), lysosomal granular membrane glycoprotein (CD63) and platelet membrane surface glycoprotein (CD41) in 45 patients with allergic purpura by flow cytometry. The expression level of anti-platelet antibody was detected by ELISA. Results The positive percentage of CD62P, CD63 and anti-platelet antibody in patients with HSP were significantly higher than those in patients in remission and control group. There was no significant difference in CD41 expression between the two groups. Purpura nephritis type CD62P, CD63 and CD41 and anti-platelet antibody expression levels compared with the mixed type, the difference was not statistically significant. Purpura nephritis and mixed type and simple skin type, CD62P higher than the latter, the difference was statistically significant; CD63, anti-platelet antibody was no significant difference. Conclusion There is abnormal humoral immunity in patients with allergic purpura. Platelet activation and allergic purpura course of change is closely related.