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1 材料与方法 药物:银杏叶本地区10月份采集,洗净凉干,75％乙醇蒸馏提纯总黄酮,黄酮含量为0.5g/ml。动物:SD系纯种大白鼠,随机分为2组。ACE试剂盒,购于北京海军总医院。ET试剂盒购于北京东亚免疫技术研究所。NO试剂盒购于南京聚力生物医学工程研究所。仪器:岛津UV-260紫外分光光度计。r免疫计数仪。 方法均按试剂盒操作说明进行。2 结果 见表 附表 银杏叶黄酮对大鼠血清ACE活动、NO含量、
1 Materials and Methods Drugs: Ginkgo biloba collected in October in this area, washed and dried, 75% ethanol distillation purification of total flavonoids, flavonoid content of 0.5g/ml. Animals: SD purebred rats were randomly divided into 2 groups. ACE kit was purchased at Beijing Naval General Hospital. The ET kit was purchased at Beijing East Asia Immunology Institute. NO kit was purchased from Nanjing Juli Biomedical Engineering Institute. Instrument: Shimadzu UV-260 UV spectrophotometer. r Immunocounter. The method was performed according to the kit operation instructions. 2 Results See Table Attached table Ginkgo biloba flavonoids on serum ACE activity, NO content,