论文部分内容阅读
目的:建立通关藤药材中通关藤苷H的HPLC含量测定方法,为完善其质量标准提供科学依据。方法:采用高效液相色谱法(HPLC),YMC ODS-H80色谱柱(4.6 mm×250 mm,4μm),乙腈-水(50∶50)为流动相,流速0.8 mL.min-1,柱温35℃,蒸发光散射检测器(ELSD)检测,雾化温度60℃,N2流速1.5 mL.min-1。结果:通关藤苷H在0.562 5~36.00μg进样量对数与峰面积对数呈良好的线性关系(r=0.999 8),平均加样回收率为99.40%(n=6),RSD 1.8%。11个产地的通关藤药材中通关藤苷H含量相差较大(按干燥品计算0.200 7%~0.862 2%)。结论:建立了通关藤药材中通关藤苷H的HPLC-ELSD含量测定方法,该方法准确、可靠,可用于通关藤药材的质量控制与评价。
OBJECTIVE: To establish a HPLC method for the determination of stannous glycoside H in Pueraria lobata. It provides a scientific basis for improving its quality standard. Methods: High performance liquid chromatography (HPLC) was used on a YMC ODS-H80 column (4.6 mm × 250 mm, 4 μm) with acetonitrile-water (50:50) as the mobile phase at a flow rate of 0.8 mL · min- 35 ℃, evaporative light scattering detector (ELSD), atomization temperature 60 ℃, N2 flow rate 1.5 mL.min-1. Results: There was a good linear relationship between the logarithm of stannous glycoside H in the range of 0.562 5 ~ 36.00μg and the logarithm of peak area (r = 0.999 8), the average recoveries were 99.40% (n = 6), RSD 1.8 %. The contents of Clenbuterol H in 11 medicinal herbs of different habitats varied greatly (from 0.200 7% to 0.862 2% on dry products). Conclusion: HPLC-ELSD method was established for the determination of Clenbuterol in Pueraria lobata. The method is accurate and reliable and can be used for the quality control and evaluation of Clemson Pharmacy.