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目的研究人血树突状细胞（ＤＣ）和细胞因子ＴＮＦ，ＧＭＣＳＦ或ＩＦＮγ联合ＤＣ对淋巴因子和ＰＨＡ激活的杀伤细胞（ＬＰＡＫ细胞）体外杀伤人肝癌细胞株ＢＥＬ７４０２的影响．方法实验分为Ｌ组（ＬＰＡＫ），Ｄ组（ＬＰＡＫ＋ＤＣ），Ｔ１组（ＬＰＡＫ＋ＤＣ＋ＴＮＦ５０００ｋＵ／Ｌ），Ｔ２组（ＬＰＡＫ＋ＤＣ＋ＴＮＦ５００ｋＵ／Ｌ），Ｇ１组（ＬＰＡＫ＋ＤＣ＋ＧＭ－ＣＳＦ５００ｋＵ／Ｌ），Ｇ２组（ＬＰＡＫ＋ＤＣ＋ＧＭ－ＣＳＦ１００ｋＵ／Ｌ），Ｉ１组（ＬＰＡＫ＋ＤＣ＋ＩＦＮγ５００ｋＵ／Ｌ）和Ｉ２组（ＬＰＡＫ＋ＤＣ＋ＩＦＮγ１００ｋＵ／Ｌ）．每组效靶细胞比分别采用５∶１和１０∶１两种．培养４８ｈ后用中性红比色法检测细胞毒活性．结果Ｌ，Ｄ，Ｔ２和Ｔ１组的细胞毒活性依次增强，各组间差异有显著性（Ｐ＜００１）．Ｇ１和Ｇ２组均高于Ｄ组（Ｐ＜００１），但Ｇ１，Ｇ２组间差异无显著性．Ｉ１，Ｉ２组与Ｄ组相比，也无显著性差异．随效靶比增加，各组细胞毒活性均相应增强．结论ＤＣ能增强ＬＰＡＫ细胞对肝癌细胞ＢＥＬ７４０２的细胞毒活性；ＴＮＦ或ＧＭＣＳＦ与ＤＣ联用，两者有协同作用；但与ＩＦＮγ联用，则无进一步增强作用
Objective To investigate the effect of human blood dendritic cell (DC) and cytokine TNF, GMCSF or IFNγ combined with DC on lymphocyte and PHA-activated killer cells (LPAK cells) in vitro to kill human hepatocellular carcinoma cell line BEL7402. Methods The experiment was divided into L group (LPAK), D group (LPAK+DC), T1 group (LPAK+DC+TNF5000kU/L), T2 group (LPAK+DC+TNF500kU/L), G1 group (LPAK+DC+GM-CSF500kU/L), G2 group (LPAK+DC+GM-CSF100kU/L ), I1 group (LPAK+DC+IFN-γ500kU/L) and I2 group (LPAK+DC+IFN-γ100kU/L). The ratio of target cells in each group was 5:1 and 10:1 respectively. The cytotoxic activity was measured by neutral red colorimetry after 48 hours culture. Results The cytotoxic activity of L, D, T2 and T1 increased in turn, and there was significant difference among the groups (P<001). G1 and G2 groups were higher than D group (P<001), but there was no significant difference between G1 and G2 groups. There was no significant difference between group I1 and group I2 compared with group D. With the increase of target-to-target ratio, the cytotoxic activity of each group increased accordingly. Conclusion DC can enhance the cytotoxic activity of LPAK cells on BEL7402 hepatoma cells; TNF or GMCSF combined with DC has a synergistic effect; but when combined with IFNγ, there is no further enhancement effect.