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目的:观察通腑汤对急性肺损伤大鼠肺组织CGRP水平的影响,探讨通腑汤治疗急性肺损伤的作用机制。方法:选取健康Wistar大鼠180只,随机分为正常对照组、模型组、地塞米松组、中药低剂量组、中药中剂量组、中药高剂量组、30只/组。尾静脉注射LPS复制ALI模型,半小时后中药各剂量组灌胃、地塞米松组腹腔注射治疗,于治疗后第1、2、6h,每组每次各10只,处死大鼠。ELISA法检测肺组织中CGRP的含量。结果:模型组CGRP含量高于正常对照组(P<0.05);地塞米松组、中药各剂量组CGRP含量均低于模型组(P<0.05);中药低剂量组CGRP含量高于地塞米松组(P<0.05);中药中、高剂量组CGRP含量与地塞米松组无差异(P>0.05);中药中、高剂量组CGRP含量均低于中药低剂量组(P<0.05);中药高剂量组CGRP含量与中药中剂量组无差异(P>0.05)。结论:通腑汤可降低ALI大鼠肺组织中CGRP的含量,高剂量组与中剂量组疗效较好。
Objective: To observe the effect of Tongfu Decoction on the level of CGRP in the lung tissue of rats with acute lung injury and to explore the mechanism of Tongfu Decoction in the treatment of acute lung injury. Methods: One hundred and eighty healthy Wistar rats were randomly divided into normal control group, model group, dexamethasone group, low dose Chinese medicine group, medium dose Chinese medicine group and high dose Chinese medicine group, 30 rats / group. After half an hour, the rats in each dose group were given intragastric administration of dexamethasone and intraperitoneal injection of dexamethasone. After 1, 2 and 6 hours of treatment, 10 rats in each group were sacrificed. Rats were sacrificed. ELISA method to detect the content of CGRP in lung tissue. Results: The content of CGRP in model group was higher than that in normal control group (P <0.05). The content of CGRP in dexamethasone group and traditional Chinese medicine group was lower than that of model group (P <0.05) (P <0.05). The contents of CGRP in medium and high dose groups were not significantly different from those in dexamethasone group (P> 0.05). The contents of CGRP in medium and high dose groups were lower than those in low dose groups (P <0.05) There was no difference in CGRP content between the high-dose group and the medium-dose group (P> 0.05). Conclusion: Tongfu Decoction can reduce the content of CGRP in the lung tissue of ALI rats, and the effect of high dose group and middle dose group is better.