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目的:探讨CD1a和E-cadherin与宫颈癌发生发展的关系及作为早期癌变生物学指标的可能性。方法:采用免疫组化SP法检测CD1a和E-cadherin在同期56例宫颈上皮内瘤变、56例宫颈鳞癌和15例正常宫颈组织中的分布及表达。结果:(1)CD1a+朗格汉斯细胞在正常宫颈、CIN和宫颈癌各组中数量逐渐减少,两两比较有显著差异(P<0.01);CIN中该细胞数量随病变严重程度减少,CINⅠ和CINⅡ、CINⅠ和CINⅢ组之间两两比较有显著差异(P<0.05),而CINⅡ与CINⅢ组间无显著差异(P>0.05);(2)E-cadher-in在正常宫颈、CIN和宫颈癌各组中的阳性表达率及强度逐渐下降,两两比较有显著差异(P<0.05);在CIN中阳性表达率及表达强度随病变严重程度呈下降趋势,CINⅠ和CINⅡ、CINⅠ和CINⅢ之间两两比较有显著差异(P<0.05),而CINⅡ和CINⅢ之间无显著差异(P>0.05);(3)宫颈组织中CD1a+朗格汉斯细胞的细胞数与E-cadherin的阳性表达率及强度呈正相关(r=0.912,P<0.05)。结论:CD1a+朗格汉斯细胞与E-cadherin可能在宫颈癌的发生、发展过程中起重要作用。
Objective: To investigate the relationship between the expression of CD1a and E-cadherin and the occurrence and development of cervical cancer and the possibility of using them as biological indicators of early canceration. Methods: The distribution and expression of CD1a and E-cadherin in 56 cases of cervical intraepithelial neoplasia, 56 cases of cervical squamous cell carcinoma and 15 cases of normal cervical tissue were detected by immunohistochemical SP method. Results: (1) The numbers of CD1a + Langerhans cells in normal cervix, cervical intraepithelial neoplasia, CIN and cervical cancer groups decreased gradually (P <0.01); the number of CIN in CIN decreased with the severity of the lesion; CINⅠ (P <0.05), but there was no significant difference between CINⅡand CINⅢgroup (P> 0.05); (2) The expression of E-cadher-in in normal cervix, CIN and CINⅢgroup was significantly different from that in CINⅡand CINⅠand CINⅢgroup The positive expression rate and intensity of cervical cancer in each group gradually decreased, with significant difference (P <0.05). The positive expression rate and expression intensity in CIN decreased with the severity of the disease. The expression of CINⅠ and CINⅡ, CINⅠ and CINⅢ (P <0.05), but there was no significant difference between CINⅡ and CINⅢ (P> 0.05); (3) The number of CD1a + Langerhans cells in cervical tissue was positively correlated with E-cadherin There was a positive correlation between expression rate and intensity (r = 0.912, P <0.05). Conclusion: CD1a + Langerhans cells and E-cadherin may play an important role in the carcinogenesis and progression of cervical cancer.