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目的比较输入性卵形疟实验室检测方法,分析他们的基因特征。方法分别用吉氏染色镜检、CareStartTM疟疾快速诊断试剂盒和巢式PCR等3种方法。对2例河南省自刚果(布)务工归来的输入性卵形疟病例进行检测,并比较检测结果。对2例外周血中疟原虫的18S rRNA基因进行测序、基因特征和同源性分析。结果2例患者均通过吉氏染色镜检观察到典型的卵形疟原虫形态,巢氏PCR均扩增出与卵形疟预期一致的特异性条带,但CareStartTM疟疾快速诊断试剂盒检测结果均为阴性。对2例外周血中疟原虫的18S rRNA基因测序,获得一条长度均为906 bp的序列,GC含量为35.4%,与已知卵形疟18S rRNA的基因序列(GenBank登录号为AB182492)同源性为99%。结论巢式PCR检测与吉氏染色镜检结果一致,CareStartTM疟疾快速诊断试剂盒检测阴性则不能排除卵形疟原虫感染。
Objective To compare the laboratory methods of imported ovipositing malaria and analyze their genetic characteristics. Methods Three methods of microscopy, CareStartTM malaria rapid detection kit and nested PCR were used respectively. Two cases of imported ovipositive malaria cases from two Congo (cloth) workers in Henan Province were tested and the test results were compared. Sequencing, gene characterization and homology analysis of 18S rRNA gene of two malarial parasites in peripheral blood were performed. Results In the two cases, the typical form of Plasmodium ovale was observed by Giemsa staining. All the specific bands were identical with those predicted by Ovary malaria test in Nestle PCR. However, both the test results of CareStartTM malaria rapid diagnostic kit Negative. The sequence of 18S rRNA gene of Plasmodium in 2 cases of peripheral blood was obtained, and a sequence with a length of 906 bp was obtained. The content of GC was 35.4%. It was homologous with the gene sequence of GenBank accession number AB182492 Sex is 99%. Conclusions The results of nested PCR and Giemsa staining are consistent. Negative detection of CareStartTM malaria rapid diagnostic kit can not rule out P. ovale infection.