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目的观察糖痹康对糖尿病大鼠坐骨神经II相代谢酶谷氨酸半胱氨酸连接酶亚基(glutamate-cysteine ligase catalytic subunit,GCLc)和谷胱甘肽-S-转移酶(glutathione s-transferase,GST)pi蛋白与mRNA的影响,探讨糖痹康对糖尿病周围神经病变大鼠坐骨神经保护作用机制。方法雄性SD大鼠,高脂饲料与小剂量链脲佐菌素(streptozotocin,STZ)联合诱发糖尿病大鼠模型,模型成功后将糖尿病大鼠随机分为5组:模型组、α-硫辛酸(alpha lipoic acid,ALA)组(0.0268g/(kg·d),糖痹康高(2.5g/kg)、中(1.25g/kg)、低(0.625g/kg)剂量组,每组10只,另将雄性SD大鼠10只设为正常组。治疗12周后测大鼠右侧坐骨神经传导速度;Western blot检测大鼠坐骨神经中GCLc和GST pi蛋白的表达;实时荧光定量PCR检测大鼠坐骨神经中GCLc和GST pi mRNA的表达。结果与模型组相比,12周后ALA组、糖痹康高、中剂量组大鼠坐骨神经传导速度明显升高(P<0.05);ALA组、糖痹康高、中剂量组GCLc和GST pi蛋白及mRNA表达均明显高于模型组(P<0.05)。结论糖痹康可通过诱导Ⅱ相代谢酶GCLc和GST pi改善糖尿病大鼠坐骨神经损伤。
Objective To observe the effects of “Tangbi Kang” on the expression of glutamate-cysteine ligase catalytic subunit (GCLc) and glutathione s-transferase , GST) pi protein and mRNA in rats with diabetic peripheral neuropathy to explore the protective mechanism of sciatic nerve in rats with diabetic peripheral neuropathy. Methods Diabetic rats were induced by a combination of high fat diet and low dose streptozotocin (STZ). The diabetic rats were randomly divided into 5 groups: model group, α-lipoic acid (0.0268g / (kg · d), Baipikang high (2.5g / kg), medium (1.25g / kg) and low (0.625g / kg) , And another 10 male Sprague-Dawley rats were set as normal group.The rats were sacrificed for 12 weeks, the right sciatic nerve conduction velocity was measured, the expression of GCLc and GST pi protein in rat sciatic nerve was detected by Western blot, and the sciatic nerve (P <0.05). Compared with the model group, the conduction velocity of sciatic nerve in ALA group and the SHR group was significantly higher than that in the untreated group (P <0.05) The expression of GCLc and GST pi protein and mRNA in high and middle dose groups were significantly higher than those in model group (P <0.05) .ConclusionShibipang can improve sciatic nerve injury of diabetic rats by inducing phase Ⅱ metabolic enzymes GCLc and GST pi.