论文部分内容阅读
为了明确春谷子Waxy基因序列变异和为品质育种提供实验依据,本研究以引进的15份春谷子为材料,利用特异PCR标记和序列比对,研究了Waxy基因序列单核苷酸多态性。结果表明:引物ex1/TSI2R、M5/M9和ex2int2/ex4在供试15份材料中分别扩增出约380 bp、1 800 bp和1 000 bp的谱带;引物ex1/ex2在利谷21、赤谷4号、A2不育度低和A2-1不育系等4份材料中均未扩增出谱带,在其余11份材料中均扩增出约1 200 bp的谱带;M2/R4在丰田501和金丰谷中未扩增出条带,在其余13份材料中均扩增出约1 500 bp的谱带。与Gen Bank粳谷KF372879的Waxy基因序列比对,共检测到419个SNPs位点和2 082个氨基酸变异。不同材料之间,总SNPs最多的为赤谷16、赤谷6号和公矮5号,分别为38、37和37,SNPs较少的有赤谷4号和利谷21,均为16;SNP多态性最多的引物为ex1/TSI2R,为159个,最少的为M5/M9,为40个;外显子共检测到47个SNPs,大白毛、A2-1不育系、丰田501、公矮5号和赤谷5号较多,分别为6、5、5、5和5个。非糯、低直链淀粉含量和糯质类型在外显子上的SNP分别为17、22和8个。
In order to clarify the sequence variation of Waxy gene in spring millet and provide experimental evidence for quality breeding, 15 WILD CHRONICIA imported from spring were used as materials to study single nucleotide polymorphism of Waxy gene sequence by using specific PCR markers and sequence alignment. The results showed that the ex1 / TSI2R, M5 / M9 and ex2int2 / ex4 primers amplified about 380 bp, 1 800 bp and 1 000 bp in 15 samples respectively. The ex1 / Chiku 4, A2 low sterility and A2-1 sterile line did not amplify the four bands of the material, in the remaining 11 were amplified about 1 200 bp band; M2 / R4 No bands were amplified in Toyota 501 and Jinfeng Valley, and bands of ~ 1500 bp were amplified in the remaining 13 materials. A total of 419 SNPs and 2 082 amino acid mutations were detected in the Waxy gene sequence of GenBank japonica rice KF372879. Among the different materials, the highest total SNPs were Red Valley 16, Red Valley 6 and Gongyi 5, which were 38, 37 and 37, respectively. There were fewer SNPs in Red Valley 4 and Li Valley 21, both of which were 16; SNP polymorphism The most abundant primers were ex1 / TSI2R with 159, with M5 / M9 of at least 40, and 47 SNPs, white hair, A2-1, Toyota 501 and Gongyi 5 And Red Valley 5 more, respectively, 6,5,5,5 and 5. The SNPs on exon of non-waxy, low amylose content and waxy type were 17,22 and 8, respectively.