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采用高效液相(HPLC)法测定杭白菊中绿原酸的含量,并通过响应面分析对其超声提取工艺进行优化。结果表明,最佳工艺为16倍量60%乙醇超声提取4次、28min/次,提取率为3.01mg/mL。
The content of chlorogenic acid in Chrysanthemum morifolium was determined by high performance liquid chromatography (HPLC) and its ultrasonic extraction process was optimized by response surface analysis. The results showed that the optimum conditions were ultrasonic extraction of 16 times amount of 60% ethanol 4 times, 28min / time, the extraction rate was 3.01mg / mL.