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目的:探讨辛伐他汀对大鼠心肌缺血再灌注后细胞凋亡及线粒体融合素2(Mitofusin 2,Mfn2)表达的影响。方法:选取32只Sprague-Dawley(SD)大鼠,随机分为假手术组、缺血再灌注模型组(模型组)、5 mg辛伐他汀组、10 mg辛伐他汀组,每组8只。5 mg辛伐他汀组、10 mg辛伐他汀组分别于术前7 d开始给予5 mg/(kg·d)、10 mg/(kg·d)的辛伐他汀灌胃,假手术组、模型组均给予等量蒸馏水灌胃。采用结扎左前降支法建立大鼠心肌缺血再灌注损伤模型。各组于造模后3 h剪取心脏组织,采用TUNEL法检测心肌细胞凋亡,Western Blot法检测p-Akt的表达,免疫组织化学法检测Mfn2的表达。结果 :与假手术组相比较,模型组心肌细胞凋亡指数、Mfn2的表达明显升高,p-Akt的表达显著降低(均P<0.05);与模型组比较,5 mg辛伐他汀组、10 mg辛伐他汀组心肌细胞凋亡指数、Mfn2的表达显著降低,p-Akt的表达显著升高(均P<0.05),并显示出明显剂量依赖性(P<0.05)。结论:辛伐他汀可有效抑制心肌缺血再灌注损伤后的心肌细胞凋亡,抑制Mfn2表达是其可能作用机制。
Objective: To investigate the effect of simvastatin on the apoptosis and the expression of Mitofusin 2 (Mfn2) after myocardial ischemia / reperfusion in rats. Methods: Thirty-two Sprague-Dawley (SD) rats were randomly divided into sham-operated group, ischemia-reperfusion model group (model group), 5 mg simvastatin group and 10 mg simvastatin group . Simvastatin 5 mg / (kg · d) and simvastatin 10 mg / (kg · d) were administered to simvastatin 5 mg and simvastatin 10 mg respectively Group were given equal volume of distilled water gavage. The model of myocardial ischemia-reperfusion injury was established by ligation of the left anterior descending artery. Cardiac tissues were harvested at 3 h after model establishment. Cardiomyocyte apoptosis was detected by TUNEL. The expression of p-Akt was detected by Western Blot and the expression of Mfn2 by immunohistochemistry. Results: Compared with the sham operation group, the apoptosis index, the expression of Mfn2 and the expression of p-Akt in the model group were significantly decreased (all P <0.05). Compared with the model group, the 5 mg simvastatin group, The apoptotic index, the expression of Mfn2, the expression of p-Akt in 10 mg simvastatin group were significantly increased (all P <0.05), and showed a dose-dependent manner (P <0.05). Conclusion: Simvastatin can inhibit myocardial ischemia-reperfusion injury of myocardial cells, inhibition of Mfn2 expression is its possible mechanism.