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目的:建立舒筋活络酒中升麻苷和5-O-甲基维斯阿米醇苷的测定方法,以控制制剂的质量。方法:采用HPLC测定舒筋活络酒中升麻苷和5-O-甲基维斯阿米醇苷的含量。Kromasil C18柱(4.6 mm×250 mm,5μm);流动相乙腈-0.05%磷酸溶液为流动相梯度洗脱;检测波长254 nm;流速1.0 mL·min-1;柱温35℃,进样量为10μL。结果:升麻苷在0.030 1～1.504 5μg,5-O-甲基维斯阿米醇苷进样量在0.040 8～2.040 0μg,与升麻苷和5-O-甲基维斯阿米醇苷总量峰面积呈良好的线性关系,平均回收率为升麻苷99.46%,RSD 2.64%,5-O-甲基维斯阿米醇苷100.45%,RSD 2.54%(n=9)。结论:本方法简便、快速、准确,可作为该制剂的定量分析方法。
OBJECTIVE: To establish a method for the determination of aconitine and 5-O-methylvishisainin in Shujinhuoluji to control the quality of preparation. Methods: The contents of aconitine and 5-O-methylvishisinin in Shujinhuo Liquor were determined by HPLC. Kromasil C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase of acetonitrile-0.05% phosphoric acid was eluted with mobile phase. The detection wavelength was 254 nm. The flow rate was 1.0 mL · min-1. 10μL. Results: The content of 5-O-methyl-Wei-Wei-Wei-Wei-Wei-Wei drug glucoside in 0.030 1 ~ 1.504 5μg, The average peak area of glycosides showed a good linear relationship with the average recoveries of 99.46%, RSD 2.64%, 100.45% and RSD 2.54% (n = 9) for 5-O-methylvitexinolide. Conclusion: The method is simple, rapid and accurate and can be used as a quantitative analysis method for this preparation.