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目的:采用正交试验设计法优选半夏泻心汤辛开苦降组药物的提取工艺。方法:以HPLC法测定的黄芩苷、盐酸小檗碱的含量以及收膏率为评价指标,采用单因素考察和正交试验设计相结合的方法,优选出最佳的提取工艺。结果:半夏泻心汤辛开苦降组药物最佳提取工艺为药材加入10倍量的65%乙醇,回流提取3次,每次2h。结论:该工艺稳定、可行,黄芩苷和盐酸小檗碱提取率较高。
OBJECTIVE: To optimize the extraction process of drugs for Banxiaxiexin Decoction by using orthogonal test design method. Methods: The content of baicalin and berberine hydrochloride and the content of berberine hydrochloride were determined by HPLC. The optimal extraction process was optimized by the combination of single factor test and orthogonal experimental design. Results: The optimal extraction technology of Banxiaxiexin Decoction was as follows: adding 10 times the amount of 65% ethanol to the medicine and refluxing for 3 times for 2 hours. Conclusion: The process is stable and feasible, and the extraction rates of baicalin and berberine are high.