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利用陈旧皮张的DNA提取技术从鸡内金、鸭内金中提取DNA,通过特异扩增DNA片段的聚合酶链式反应(PCR),以线粒体DNA细胞色素b(cyt-b)通用引物中的L14841和H15149为引物扩增DNA片段,将扩增后的DNA 用双脱氧链终止法测定其序列,所测序列分别与文献报道的鸡、鸭序列一致。所得结果证明鸡内金的DNA序列与鸭内金的DNA序列有明显差异,以此能准确区分鸡内金和鸭内金。开创了将分子遗传学技术引入动物药材鉴定的先例,增加了动物药材的鉴定技术,使其鉴定手段提高到分子水平,以增加鉴定结果准确性。并探讨了将此技术引入以动物组织、器官入药的动物药材鉴定中的可行性。
DNA was extracted from chicken’s inner gold and duck’s inner gold using an old-skinned DNA extraction technique and polymerase chain reaction (PCR) of a specific amplified DNA fragment was used in the mitochondrial DNA cytochrome b (cyt-b) universal primer. L14841 and H15149 were primers to amplify DNA fragments. The amplified DNA was sequenced using the dideoxy chain termination method. The sequences were identical to the chicken and duck sequences reported in the literature. The obtained results showed that there was a significant difference between the DNA sequence of chicken guinea pig and the DNA sequence of duck guinea pig, in order to distinguish chicken inner gold and duck inner gold accurately. It created a precedent for the introduction of molecular genetics technology into the identification of animal medicines, increased the identification technology of animal medicines, and increased its identification methods to the molecular level to increase the accuracy of identification results. The feasibility of introducing this technology into the identification of animal medicines for animal tissues and organs was explored.