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从皮革厂、畜肉市场、屠宰场等处采样,分离得到49株明胶水解活性的细菌。经活性平板初筛、牛皮消化试验和活性测定,筛选得到一株胶原蛋白酶活性为35.97u/mL的菌株。经形态学观察、生理生化特性和16SrDNA序列分析,鉴定该菌株为短小芽孢杆菌。
From the tannery, meat market, slaughterhouse, etc. sampling, isolated 49 gelatinolytic activity of bacteria. After screening by active plate, digestion test and activity assay, a strain with collagenase activity of 35.97u / mL was screened out. Morphological observation, physiological and biochemical characteristics and 16S rDNA sequence analysis showed that the strain was Bacillus pumilus.