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目的研究遗传印记基因PEG10在结肠癌、癌旁组织、正常结肠组织中的表达及意义。方法收集中国人民解放军第一七四医院2015年1月-2015年9月40例结肠癌、癌旁组织,20例正常结肠组织标本,通过免疫组织化学及蛋白印迹法检测PEG10的细胞定位及其表达差异。结果 PEG10细胞主要分布于细胞质,在结肠癌中呈过表达,其表达明显高于癌旁组织、正常结肠组织,差异有统计学意义(P<0.01)。结论 PEG10可能参与结肠癌发生、发展,但其具体作用机制仍需进一步探讨。
Objective To study the expression and significance of genetic imprinting gene PEG10 in colon cancer, adjacent non-cancerous tissues and normal colon tissues. Methods Collected 40 cases of colon cancer, paracancer tissue and 20 cases of normal colon tissue from 174th Hospital of Chinese People’s Liberation Army from January 2015 to September 2015, and the cellular localization of PEG10 was detected by immunohistochemistry and Western blot Differences in expression. Results PEG10 cells were mainly distributed in the cytoplasm and overexpressed in colon cancer, which was significantly higher than that in paracancerous tissues and normal colon tissues (P <0.01). Conclusion PEG10 may be involved in the occurrence and development of colon cancer, but the specific mechanism of action still needs to be further explored.