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目的建立同时测定鸦胆子油中棕榈酸、硬脂酸、油酸和亚油酸4种成分含量的方法。方法采用毛细管气相色谱法,甲酯化后测定鸦胆子油中棕榈酸、硬脂酸、油酸和亚油酸的含量。色谱柱:DB-17弹性石英毛细管柱(30 m×0.25 mm×0.25μm),检测器:氢火焰离子化检测器(FID),柱温恒温:200℃(保持11 min),进样口温度:250℃,检测器温度:250℃,流速:1.0 mL.min-1,分流比:20∶1,内标物:苯甲酸苯酯。结果棕榈酸、硬脂酸、油酸和亚油酸的线性分别为74～738 mg.L-1(r=0.999 6)、51～509 mg.L-1(r=0.999 1)、741～7 410 mg.L-1(r=0.999 1)和265～2 650 mg.L-1(r=0.999 1);各成分的平均回收率(n=9)分别为94.6%、94.2%、94.2%和95.3%。结论此方法可为鸦胆子油的质量评价提供依据。
Objective To establish a method for the simultaneous determination of palmitic acid, stearic acid, oleic acid and linoleic acid in Brucea javanica oil. Methods The contents of palmitic acid, stearic acid, oleic acid and linoleic acid in Brucea javanica oil were determined by capillary gas chromatography after methyl esterification. Column: DB-17 elastic quartz capillary column (30 m × 0.25 mm × 0.25 μm), detector: hydrogen flame ionization detector (FID), column temperature constant temperature: 200 ℃ for 11 min, inlet temperature : 250 ° C, detector temperature: 250 ° C, flow rate: 1.0 mL.min-1, split ratio: 20: 1, internal standard: phenyl benzoate. Results The linearities of palmitic acid, stearic acid, oleic acid and linoleic acid were 74 ~ 738 mg · L -1 (r = 0.999 6), 51 ~ 509 mg · L -1 (r = 0.999 1), 741 ~ (N = 9) were 94.6%, 94.2% and 94.2%, respectively. The average recoveries of each component were 7 410 mg.L-1 (r = 0.999 1) and 265-2 650 mg.L- % And 95.3%. Conclusion This method can provide the basis for the quality evaluation of Brucea javanica oil.