论文部分内容阅读
目的 检测细胞因子IL 2、IL 10、TNF α在单纯疱疹病毒性脑炎 (HSE)中的表达和变化 ,探讨细胞因子IL 2、IL 10、TNF α在HSVE发病机制中的作用。方法 使用逆转录—聚合酶链反应 (RT PCR)检测颅内感染单纯疱疹病毒 1型 (HSV1)的小鼠在感染后及使用无环鸟苷 (ACV)治疗后细胞因子IL 2、IL 10、TNF α变化及病理变化。结果 HSV1感染后出现脑内出血坏死性的病理改变 ,IL 2、IL 10、TNF α均明显上升 ;无环鸟苷治疗好转后脑内病理变化改善 ,IL 2保持稳定 ,IL 10继续上升 ,TNF α显著下降。结论 在小鼠HSVE急性期TH1型及TH2型反应同时被激活 ,发挥抗病毒作用 ,并以TH1型反应为主 ;在HSVE恢复期以TH2型反应为主 ,并抑制体内免疫反应的扩大 ;3种细胞因子的动态变化反映出机体免疫调节的动态平衡 ,并可反映HSVE的预后 ,早期应用ACV治疗HSVE确有极其明显的抗病毒治疗作用。
Objective To investigate the expression and changes of cytokines IL 2, IL 10 and TNF α in herpes simplex encephalitis (HSE) and to explore the role of cytokines IL 2, IL 10 and TNF α in the pathogenesis of HSVE. Methods RT-PCR was used to detect the intracerebral infection of herpes simplex virus type 1 (HSV1) mice after infection and after acyclovir (ACV) treatment. The levels of IL 2, IL 10, TNFα changes and pathological changes. Results Pathological changes of hemorrhagic necrosis were observed after HSV1 infection. The levels of IL 2, IL 10 and TNF α were significantly increased. After the treatment of acyclovir, the pathological changes of brain were improved, IL 2 kept stable, IL 10 continued to rise, and TNF α was significantly increased decline. Conclusions TH1-type and TH2-type responses are activated simultaneously in the acute phase of HSV in mice, which play an antiviral role and predominantly TH1-type responses. In the recovery phase of HSVE, TH2-type responses are predominant and inhibit the expansion of the immune response in vivo. 3 The dynamic changes of cytokines reflect the dynamic balance of immune regulation, and can reflect the prognosis of HSVE, early application of ACV treatment of HSVE does have a very clear anti-viral treatment.