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目的探讨survivin抗体及其基因表达在肺癌诊断中的应用价值。方法 2013年1月至2013年5月期间,本院诊治的24例肺癌患者(观察组),以及24例健康者(对照组),作为研究对象,采集外周血液标本,通过ELISA法、RT-PCR法,检测两组血清中survivin抗体及survivin mRNA表达水平。结果与对照组相比,观察组血清中survivin抗体及survivin mRNA表达水平均明显升高,P<0.05,差异有统计学意义,并且血清survivin抗体表达与survivin mRNA表达呈正相关。结论 survivin抗体及其基因表达检测有利于肺癌的临床诊断,值得临床推广。
Objective To investigate the value of survivin antibody and its gene expression in the diagnosis of lung cancer. Methods From January 2013 to May 2013, 24 patients with lung cancer (observation group) and 24 healthy controls (control group) were included in the study. Peripheral blood samples were collected and analyzed by ELISA, RT- PCR method was used to detect the expression of survivin and survivin mRNA in serum of two groups. Results Compared with the control group, the expression levels of survivin mRNA and survivin mRNA in the observation group were significantly increased, P <0.05, the difference was statistically significant, and the expression of survivin antibody was positively correlated with the expression of survivin mRNA. Conclusion The detection of survivin antibody and its gene expression is helpful for the clinical diagnosis of lung cancer and is worthy of clinical promotion.