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目的通过分子流行病学调查研究兰州地区婴幼儿病毒性腹泻的病原学特点。方法采用酶联免疫吸附试验(ELISA)及逆转录聚合酶链反应(RTPCR),对2003年7月至2004年6月兰州地区收集的624例婴幼儿腹泻粪便标本随机抽取271例进行轮状病毒(RV)、杯状病毒(HuCV)及星状病毒(AstV)检测。结果在271例标本中共检出RV感染153例(56.46%),其中G394例(61.44%),G24例(2.61%),G93例(1.96%),未发现G1、G4型和混合感染;在随机抽取的69例G分型阳性标本中,检出P[8]型28例(40.58%);RV的感染对象主要为6～23月龄的婴幼儿,发病高峰在10、11月份(86.27%、73.81%)。在118例RVELISA阴性标本中检出HuCV感染13例(11.02%),其中诺如病毒(NLV)GⅡ型11例,札如病毒(SLV)2例,未检出NLVGⅡ型,发病年龄1～18个月(11.31±4.53个月);同时检出AstV感染7例(5.93%),发病年龄4～12个月(8.27±2.69)个月,其中有1例合并有SLV感染,另有1例为迁延性腹泻。HuCV和AstV感染均未表现出明显的季节性。结论RV是兰州婴幼儿病毒性腹泻的主要病原,其流行的主要血清型为G3型,HuCV和AstV亦是重要病原之一。
Objective To investigate the etiological characteristics of infant diarrhea in Lanzhou through molecular epidemiological investigation. Methods A total of 624 infants and young children with diarrhea stool collected in Lanzhou from July 2003 to June 2004 were randomly selected from 271 patients for rotavirus infection by enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RTPCR) (RV), Calicivirus (HuCV) and Astrovirus (AstV). Results A total of 153 cases (56.46%) of RV infections were detected in 271 specimens, among which G394 cases (61.44%), G24 cases (2.61%) and G93 cases (1.96%) did not find G1, G4 and mixed infections. Of the 69 cases of G-type positive samples randomly selected, 28 cases (40.58%) of P [8] were detected. The main infection target of RV was 6-23 months old infants and young children. The peak incidence peak was in October and November (86.27% %, 73.81%). In 118 RVELISA negative specimens, 13 (11.02%) were detected in HuCV infection. Among them, 11 cases of Norovirus (GVII) and 2 cases of ZV (NLVG) Months (11.31 ± 4.53 months) .At the same time, 7 cases (5.93%) of AstV infections were detected and the age of onset was 4 to 12 months (8.27 ± 2.69) months, of which 1 case had SLV infection and 1 case For persistent diarrhea. HuCV and AstV infection showed no obvious seasonal. Conclusion RV is the main pathogen of infantile viral diarrhea in Lanzhou. The predominant serotype is G3, and HuCV and AstV are also one of the important pathogens.