目的 :探讨联合培养的血管平滑肌细胞 (VSMC)对内皮细胞 (EC)粘附能力的影响。 方法 :应用免疫荧光细胞化学、激光共聚焦扫描显微镜和图像分析等技术 ,以静态条件下单独培养的 EC为对照组 ,观察与 VSMC联合培养的 EC细胞外基质 Fn,L n和 Col 的构筑方式及其含量的变化。结果 :与单独培养的 EC相比 ,联合培养的 EC细胞外基质各组分的构筑有明显的变化 ,且含量也发生变化 ,Fn,L n增多 ,Col 减少。结论 :与 VSMC联合培养的 EC的粘附能力可能增强 ,为改进血管 EC种植的组织工程技术提供了部分实验依据。 Objective: To investigate the effect of co-cultured vascular smooth muscle cells (VSMCs) on the adhesion of endothelial cells (ECs). Methods: Immunofluorescence cytochemistry, confocal laser scanning microscopy and image analysis techniques were used to study the construction of EC extracellular matrix (Fn, Ln and Col) co-cultured with VSMC under the static condition. And its content changes. Results: Compared with the EC cultured alone, the ECs of the co-cultured ECs had obvious changes in the composition of the extracellular matrix. The content of Fn and Ln increased and Col decreased. Conclusion: The adhesion ability of EC cultured with VSMC may be enhanced, which provides some experimental evidences for improving the tissue engineering technology of vascular EC implantation.