以秦美猕猴桃茎段为外植体,诱导愈伤组织并进行猕猴桃再分化及生根试验。在分化培养基中分别添加不同浓度的铜离子进行筛选,以确定适于猕猴桃愈伤组织分化的最佳铜离子浓度。结果表明:再分化培养基中添加1.0 mg.L-1的铜离子时,可明显提高猕猴桃愈伤组织绿苗再分化率及生根率。但当铜离子浓度超过2.0 mg.L-1时,猕猴桃愈伤组织绿苗再分化率显著降低。 The stems of Actinidia chinensis were used as explants to induce callus and conduct the re-differentiation and rooting of Actinidia chinensis. Different concentrations of copper ions were added to the differentiation medium for screening, so as to determine the optimal copper ion concentration suitable for the differentiation of kiwifruit callus. The results showed that the addition of 1.0 mg.L-1 copper ions to the redifferentiation medium could significantly improve the redifferentiation rate and rooting rate of kiwifruit callus. However, when the Cu2 + concentration exceeded 2.0 mg.L-1, the redifferentiation rate of green shoots in kiwifruit callus decreased significantly.