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对利用cDNA-AFLP技术所获得的茶树低温诱导差异表达片段TDF,通过RACE方法获得含完整编码区序列的茶树RAV基因cDNA克隆,其开放阅读框编码361个氨基酸,包含两个保守的结构域AP2和B3,与多种植物RAV蛋白具有高度同源性。qRT-PCR分析表明,茶树RAV基因受低温、乙烯、NaCl等上调表达,最大表达量分别是诱导前的5.8、10.0和1.9倍。在成熟叶片、芽、嫩茎中RAV基因表达量相近,花蕾和嫩根中表达较低,而在种子中不表达。推测该基因在组织中的表达受到严格控制以及在响应非生物胁迫中发挥重要作用。
The differential expression fragment TDF was induced by low temperature of cDNA-AFLP, and the cDNA clone of RAV gene with complete coding region was obtained by RACE. The open reading frame of the cDNA clone contained 361 amino acids, including two conserved domains AP2 And B3, with a wide range of plant RAV protein has a high degree of homology. qRT-PCR analysis showed that the RAV gene of tea tree was up-regulated by low temperature, ethylene, NaCl and so on, the maximum expression level was 5.8,10.0 and 1.9 times before induction. The expression of RAV gene was similar in mature leaves, buds and tender stems, but lower in flower buds and tender roots, but not in seeds. It is speculated that the expression of this gene in tissues is strictly controlled and plays an important role in response to abiotic stress.