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为验证枯草芽胞杆菌与氟环唑联用对禾谷镰孢霉的增效作用,用平板倒扣法和固体平板扩散法—打孔法测定菌药联用后枯草芽胞杆菌挥发性物质、抗真菌蛋白对禾谷镰孢霉的抑菌活性,观察抗真菌蛋白对禾谷镰孢霉菌丝形态的影响,并用刚果红染色法测定菌药联用后枯草芽胞杆菌纤维素酶活性。结果表明:菌药联用对禾谷镰孢霉的最高抑菌活性可达74.44%,表现增效和持效作用;枯草芽胞杆菌挥发性物质和抗真菌蛋白分别与氟环唑联用均可增强禾谷镰孢霉抑菌活性;菌药联用后枯草芽胞杆菌纤维素酶活性提高。研究表明枯草芽胞杆菌与氟环唑联用的增效机制是增强枯草芽胞杆菌挥发性物质对禾谷镰孢霉的抑菌活性,抗真菌蛋白活性的提高加强了禾谷镰孢霉菌丝溶解的程度,纤维素酶活性的增强提高了禾谷镰孢霉空间竞争作用和生物防治潜能。
In order to verify the synergistic effect of Bacillus subtilis and epoxiconazole on Fusarium graminearum, the volatile compounds of Bacillus subtilis after combined use of bacterium and medicine were determined by the plate down-draw method and solid plate diffusion method, Fungal protein against Fusarium graminearum antibacterial activity observed antifungal protein Fusarium graminearum filamentous morphology, and Congo red staining method for the determination of bacilli Bacillus subtilis cellulase activity. The results showed that the highest antibacterial activity of Fusarium graminearum was 74.44%, which showed synergism and persistence effect. Bacillus subtilis volatiles and antifungal protein could be combined with epoxiconazole respectively Enhance the antibacterial activity of Fusarium graminearum; Bacillus subtilis cellulase activity increased after combined use of bacteria and drugs. Studies have shown that Bacillus subtilis and epoxiconazole synergistic mechanism is enhanced Bacillus subtilis volatile substances against Fusarium graminearum antibacterial activity, antifungal protein activity increased Fusarium graminearum mycelium dissolved The enhancement of cellulase activity enhances the space competition and biological control potential of Fusarium graminearum.