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采用冷冻干燥(冻干)技术对细菌进行长期保存已得到了广泛的应用,是迄今最常用和最有效的细菌保存方法。目前,我国布鲁杆菌兽用生产和检验菌种亦是采用冻干方法进行长期保存的,《兽用生物制品质量标准》中规定布鲁杆菌菌种的保存期为10年。本文对1986年以来实验室采用冻干低温保存的布鲁杆菌干燥物进行观察和实验分析。1材料与方法1.1菌株系1984年以来内蒙分离的布鲁杆菌,
Long-term preservation of bacteria by freeze-drying (lyophilization) has been widely used and is by far the most commonly used and most effective method of bacterial preservation. At present, the brucella mycotoxin production and testing strains are also used for long-term preservation by freeze-drying method, “veterinary biological products quality standards” provisions of Brucella sp. Shelf life of 10 years. In this paper, the laboratory since 1986, the use of freeze-dried low temperature preservation of Brucella dried substance observed and experimental analysis. 1 Materials and Methods 1.1 strains of Brucella bacteria isolated in Inner Mongolia since 1984,