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目的 揭示肝素辅助因子Ⅱ（ Ｈ ＣⅡ） 体内抗凝的生理意义，探讨妊娠高血压综合征（ 妊高征） 并发血栓形成时的部分发病机制。方法 ①采用发色底物法测定１８ 名正常人，１８ 名正常妊娠妇女及２９ 例妊高征患者分娩前、后血浆 Ｈ ＣⅡ活性；②用 Ｗｅｓｔｅｒｎ 印迹法检测２９ 例妊高征患者分娩前、后血浆 Ｈ ＣⅡ抗原；③免疫荧光定位检测 Ｈ ＣⅡ在胎盘组织上的分布。结果 妊高征患者产前血浆 Ｈ ＣⅡ活性及抗原含量显著降低，其降低程度与妊高征分期相关，产后恢复接近正常；在正常及病理妊娠胎盘未检测到 Ｈ ＣⅡ。结论 ① Ｈ ＣⅡ参与妊高征患者妊娠期高凝状态下凝血酶的灭活过程；②胎盘并非 Ｈ ＣⅡ蛋白的锚定部位；③血浆 Ｈ ＣⅡ可作为反映妊高征病情严重度或预后判定的指标之一。
Objective To reveal the physiological significance of anticoagulation of heparin cofactor Ⅱ (H C Ⅱ) in vivo and to explore the pathogenesis of thrombosis in patients with pregnancy induced hypertension (PIH). Methods ① Plasma H CⅡ activity before and after labor in 18 normal pregnant women, 18 normal pregnancy women and 29 pregnancy induced hypertension syndromes were measured by chromogenic substrate method; ② The plasma H CⅡ activity in preterm labor, After the plasma H C Ⅱ antigen; ③ immunofluorescence localization of H C Ⅱ in placental distribution. Results Prenatal plasma H CⅡ activity and antigen content were significantly decreased in patients with PIH. The extent of H CⅡin plasma was related to the stage of PIH. The postnatal recovery was almost normal. H CⅡ was not detected in normal and pathological placenta. Conclusions ① H CII is involved in the inactivation process of thrombin during pregnancy hypercoagulable state in patients with PIH. ②The placenta is not the anchoring site of H CⅡ protein. ③ Plasma H CⅡ can be used as a criterion to judge the severity or prognosis of PIH One of the indicators.