论文部分内容阅读
目的:探讨粒-巨噬细胞集落刺激因子(granulocyte-macrophage colonv stimulating factor,GM-CSF)基因修饰的肿瘤细胞疫苗治疗前后肺癌患者外周血中DC两个不同亚群(DC1,DC2)的比例、治疗前后比例变化与临床病理特征的相关性及对生存的影响。方法:对74例接受GM-CSF基因修饰的肿瘤细胞疫苗(GM-CSF modified tumor cell vaccine,GVAX)治疗的肺癌患者,采用流式细胞技术检测患者治疗前后外周血DC及淋巴细胞亚群的比例,分析治疗前后DC比例的变化、治疗前后DC比例与治疗前血清标志物及免疫细胞的关系、治疗前后DC比例对肺癌患者生存的影响。结果:接受GVAX疫苗治疗后外周血DC1与DC2比例无明显变化(P_(DC1)=0.786,P_(DC2)=0.779);神经烯醇化酶(NSE)水平升高组中的DC亚群比例高于NSE水平正常组;治疗后DC2比例与治疗前Treg呈负相关;对于早期肺癌患者,治疗后DC2比例低于均值者的生存时间比高于均值者的生存时间明显延长。结论:治疗后DC2比例可作为早期患者GVAX疫苗疗效及判断预后的免疫指标,其对预后的影响可能与患者外周血Treg比例有一定的相关性
Objective: To investigate the ratio of DCs (DC1, DC2) in peripheral blood of patients with lung cancer before and after treatment with granulocyte-macrophage colony stimulating factor (GM-CSF) gene modified tumor cell vaccine, The relationship between the changes of the ratio before and after treatment and the clinicopathological features and its impact on survival. Methods: The proportion of peripheral blood DC and lymphocyte subsets in 74 patients with lung cancer treated with GM-CSF modified tumor cell vaccine (GVAX) was measured by flow cytometry The changes of DC proportion before and after treatment, the relationship between the proportion of DC before and after treatment and serum markers and immune cells before treatment, and the effect of DC proportion before and after treatment on the survival of patients with lung cancer were analyzed. Results: There was no significant difference in the proportion of DC1 to DC2 in peripheral blood after receiving GVAX vaccine (P_ (DC1) = 0.786, P_ (DC2) = 0.779). The proportion of DC subsets in the group with elevated neuroenoenolase (NSE) In normal NSE group, the proportion of DC2 after treatment was negatively correlated with pre-treatment Treg. For patients with early-stage lung cancer, the survival time was significantly longer than those with mean DC2 after treatment. Conclusion: The proportion of DC2 after treatment can be used as an immunological indicator of curative effect and prognosis of GVAX vaccine in early stage, and its effect on prognosis may have a certain correlation with Treg proportion in peripheral blood of patients