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目的:观察补肾活血中药对SD大鼠慢性高眼压(elevated intraocular pressure,EIOP)模型视网膜神经节细胞凋亡相关基因Bcl-2、Bax表达的影响。方法:采用烙闭上巩膜静脉法,制作大鼠慢性EIOP模型,随机分为模型对照组,补肾活血高剂量组、中剂量组、低剂量组,空白对照组。连续灌胃8周,并于8周末处死大鼠,观察其对EIOP大鼠视网膜神经节细胞(retinal ganglion cells,RGCs)凋亡基因的影响。结果:补肾活血中药可上调大鼠EIOP视网膜神经节细胞抗凋亡基因Bcl-2、抑制凋亡促进基因Bax。结论:补肾活血中药可调控大鼠EIOP视网膜神经节细胞凋亡基因的表达。
OBJECTIVE: To observe the effects of Bushen Huoxue Chinese Herbs on apoptosis-related genes Bcl-2 and Bax of retinal ganglion cells in the model of chronic intraocular pressure (EIOP) in SD rats. Methods: A chronic EIOP model was established by the method of branching and closing the scleral vein. The rats were randomly divided into model control group, high-dose tonifying kidney and blood circulation group, medium dose group, low dose group and blank control group. The rats were sacrificed at the end of the 8th week, and the effect on the apoptosis gene of retinal ganglion cells (RGCs) in EIOP rats was observed. Result: Bushen Huoxue Herbs can up-regulate the anti-apoptotic gene Bcl-2 and inhibit apoptosis-promoting gene Bax in rat EIOP retinal ganglion cells. Conclusion: Bushen Huoxue Herbal Medicine can regulate the expression of apoptosis gene in rat retinal ganglion cells.