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延缓性排斥反应 (delayedxenograftrejection ,DXR)是进行异种器官移植亟待解决的问题之一 .在DXR过程中 ,核心事件之一是核转录因子NF κB的激活 .人腺病毒 5 (Ad5 )的早期转录产物E1A蛋白可抑制以NF κB为核心的信号转导系统 .利用转基因技术向小鼠的受精卵导入了E1A基因 ,PCR和Southern印迹检测了 4 4只仔鼠 ,其中有 8只整合了E1A基因 .RT PCR检测发现 ,3只小鼠F1代的心、肝、肾等脏器都有E1AmRNA的表达 ,小鼠表型正常 .通过尾静脉向转基因阳性小鼠体内注射人灭活血清 ,模拟DXR发生的生理过程并用免疫荧光检测脏器细胞表面炎症分子E 选择素的表达水平 .结果表明 ,E1A基因的转导显著抑制了小鼠脏器细胞表面E 选择素的表达 ,为解决DXR的发生提供了可行的途径
Delayed rejection (DXR) is one of the most urgent problems in xenotransplantation.During DXR, one of the core events is the activation of the nuclear transcription factor NF κ B. The early transcript of human adenovirus 5 (Ad5) E1A inhibits NFκB signal transduction system.E1A gene was introduced into mouse zygotes by transgenic technology and 4 4 offspring were detected by PCR and Southern blotting, of which 8 were integrated with E1A gene. The results of RT-PCR showed that E1A mRNA was expressed in heart, liver and kidney of 3 mice, and the phenotype of mice was normal.The transgene-positive mice were injected with human inactivated serum through the tail vein to simulate the occurrence of DXR And the expression of E-selectin on visceral cell surface was detected by immunofluorescence.The results showed that the transduction of E1A gene significantly inhibited the expression of E-selectin on the surface of mouse organ cells and provided a solution to the occurrence of DXR Feasible way