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一、序言目前国内薄层色谱的定量方法,一般仍用手工操作,手续繁杂,所需时间也较长,应尽快生产国外已广泛使用的薄层扫描仪。但国外常用的薄层扫描仪,是根据被测物质的光学性质进行检测的。其使用性能随被测物质而异,结构也比较复杂,不易制造。1967年Padley提出了薄层棒火焰离子化检测器(FID)扫描仪,用表面涂吸附剂的ф0.9×200毫米的石英棒代替通常的薄层层析板,将样品点于棒端,按一般的薄层色谱法展开分离。展开后挥发除去展开剂,被分离的样品组份斑点留在棒上,然后直接通过FID的火焰进行定量分析。该仪器较过去的光学扫描仪具有结构及操作简单,制造较易,应用范围广及线性范围宽等特点。本文对薄层棒色谱法和FID扫描仪的操作方法和操作条件进行了研究。
First, the preamble At present, the quantitative method of domestic TLC, the general still manual operation, complicated procedures, the time required is longer, should be produced as soon as possible abroad has been widely used thin-layer scanners. However, commonly used foreign thin-layer scanners, is based on the optical properties of substances tested. The use of performance varies with the test substance, the structure is also more complex, not easy to manufacture. 1967 Padley proposed a thin rod flame ionization detector (FID) scanner, with a surface coated with ф 0.9 × 200 mm quartz rod instead of the usual TLC, the sample point in the rod end, According to the general TLC separation. After deployment, the developer is volatilized to remove the developer. The separated sample components remain on the rod and then pass through the flame of the FID for quantitative analysis. Compared with the past optical scanner, the instrument has the advantages of simple structure and operation, easy manufacture, wide application range and wide linear range. In this paper, thin rod chromatography and FID scanner operating methods and operating conditions were studied.