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目的:观察胆管癌组织及血清中诱导受体3(decoy receptor 3,DcR3)蛋白的表达及其临床价值。方法:采用免疫组化S-P法检测45例胆管癌、15例癌旁胆管正常组织中DcR3蛋白的表达,ELISA法检测31例胆管癌及18例胆道良性疾病患者和28例正常人外周血清中DcR3的水平。结果:45例胆管癌组织中DcR3阳性表达29例,阳性率为64.4%,胆管正常组织中无阳性表达。DcR3的表达与肿瘤临床分期、肿瘤浸润和转移有关(P<0.05)。胆管癌患者及胆管良性疾病患者血清DcR3水平分别为152.2535.94 pg/ml,98.35 14.27 pg/ml,均高于正常人。胆管癌患者与胆道良性疾病患者血清DcR3水平相比差异有显著性(P<0.01)。结论:DcR3在胆管癌组织中表达增高。DcR3的表达与胆管癌的发生、发展以及转移有关,可成为治疗胆管癌的一个新靶点。血清DcR3的检测对胆管癌的诊断有一定的临床价值。
Objective: To observe the expression of decoy receptor 3 (DcR3) protein and its clinical value in cholangiocarcinoma tissue and serum. Methods: Immunohistochemical SP method was used to detect the expression of DcR3 protein in 45 cases of cholangiocarcinoma and 15 cases of adjacent normal bile duct tissues. ELISA was used to detect the expression of DcR3 in peripheral blood of 31 cases of cholangiocarcinoma and 18 cases of biliary benign disease and 28 cases of normal controls s level. Results: The positive expression of DcR3 in 45 cholangiocarcinoma tissues was 29 cases, the positive rate was 64.4%. There was no positive expression of DcR3 in cholangiocarcinoma tissues. The expression of DcR3 was correlated with clinical stage, tumor invasion and metastasis (P <0.05). Serum levels of DcR3 in patients with cholangiocarcinoma and benign diseases of biliary tract were 152.2535.94 pg / ml and 98.35 14.27 pg / ml, respectively, which were all higher than those in normal people. There was a significant difference in serum DcR3 levels between patients with cholangiocarcinoma and benign diseases of biliary tract (P <0.01). Conclusion: DcR3 expression in cholangiocarcinoma increased. DcR3 expression and the occurrence, development and metastasis of cholangiocarcinoma may become a new target for the treatment of cholangiocarcinoma. Serum DcR3 detection of cholangiocarcinoma diagnosis has some clinical value.