【摘 要】
：
利用玉米转录因子ZmDREB3基因(Gene ID:EU964828.1)构建了Ubiquitin启动子驱动的植物表达载体PGM0229-ZmDREB3-EP-SPS,以EPSPS基因为抗性筛选标记,通过花粉管通道法将农杆菌E
【机 构】
：
河北农业大学生命科学学院,北京市农林科学院北京农业生物技术研究中心,
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利用玉米转录因子ZmDREB3基因(Gene ID:EU964828.1)构建了Ubiquitin启动子驱动的植物表达载体PGM0229-ZmDREB3-EP-SPS,以EPSPS基因为抗性筛选标记,通过花粉管通道法将农杆菌EHA105介导的植物表达载体转化到玉米自交系吉444、Mo17中,通过喷洒350mg/L草甘膦除草剂筛选,得到7株草甘膦抗性植株,用PCR检测得到2个同时整合EPSPS标记基因和ZmDREB3目的基因的转基因株系,用PCR-Southern进一步验证,结果呈阳性。以上结果证明外源基因已经被整合到玉米基因组中。
The Ubiquitin promoter-driven plant expression vector PGM0229-ZmDREB3-EP-SPS was constructed by using the ZmDREB3 gene (Gene ID: EU964828.1). The EPSPS gene was screened for resistance. Agrobacterium tumefaciens EHA105 The plant expression vector was transformed into maize inbred line JI444 and Mo17 and then selected by spraying 350 mg / L glyphosate herbicide to obtain 7 glyphosate resistant plants. Two EPSPS markers Gene and transgenic ZmDREB3 gene lines, further verified by PCR-Southern, the result was positive. The above results prove that foreign genes have been integrated into the maize genome.
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