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以四倍体葡萄为父本,与二倍体无核葡萄杂交,取其胚珠培养获得三倍体植珠。胚珠培养的三倍体胚发育率为3.0%~8.3%,成苗率为0.5%~5.0%。根尖细胞染色体计数57条。在圃场内栽培的三倍体植株花而不稔。用GA325mg/L处理可获得良好的果穗。试验表明,无核葡萄胚珠培养是葡萄三倍体育种的一条新途径。
The tetraploid grapes as the male parent, crossed with the diploid seedless grape, take the ovule culture to obtain triploid pearl. The diploid embryo development rate of ovule culture was 3.0% ~ 8.3% and the seedling rate was 0.5% ~ 5.0%. Root tip cell chromosome counts 57. The triploid plants cultivated in the nursery field bloom without disturbance. GA325mg / L treatment can get a good ear. Experiments show that the seedless grapes ovule culture is a new way of grape triploid breeding.