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目的:研究红外光谱(IR)是否可以反映枳实中黄酮类成分的种类差异。方法:HPLC确定枳实中黄酮类成分的种类;傅里叶变换红外光谱测定仪测定IR,以对照药材的IR为基准,红外光谱测定仪软件计算其他样品的IR与对照药材的相关系数。结果:11个批次枳实按主要黄酮类成分种类分成两类,8个批次含有新橙皮苷和柚皮苷其中包括枳实对照药材,3个批次不含有新橙皮苷和柚皮苷。含有新橙皮苷和柚皮苷的7个批次样品的IR与对照药材比较相关系数在0.90以上,含有新橙皮苷和柚皮苷的它们的IR存在高度的相关性,而不含有新橙皮苷和柚皮苷的3个批次枳实它们的IR与对照药材相比相关系数小于0.50。结论:IR可以判断枳实中黄酮类成分种类差异。
Objective: To study whether infrared spectroscopy (IR) can reflect the difference of flavonoids in Gorgon. Methods: The flavonoids were determined by HPLC. The Fourier transform infrared spectrometer was used to determine the IR. IR of the reference drug was used as reference. The infrared spectrometer software was used to calculate the correlation coefficient between the IR of other samples and the reference drug. RESULTS: 11 batches of quail were divided into two groups according to the main flavonoids. Eight batches contained neohesperidin and naringin, which included quail control, and three batches did not contain neohesperidin and pomelo. Skin glycosides. The correlation coefficient between the IR samples of the seven batches containing neohesperidin and naringin and the control drug was above 0.90, and there was a high correlation between their IRs containing neohesperidin and naringin, but no new ones. Three batches of hesperidin and naringin bred their IR with a correlation coefficient of less than 0.50 compared to the reference drug. Conclusion: IR can be used to determine the differences of flavonoids in moromi.