Objective:To evaluate the anticancer activity of the methanolic extract of Cyathula prostrata in Ehrlich ascites carcinoma(EAC)-bearing mice with methotrexate as positive control in the advanced stage of tumorigenesis.Methods:EAC was induced in swiss albino mice by injecting10~6 cell/mL of tumor cell suspension intraperitoneal.The methanolic extract of Cyathula prostrata effect on the tumor cell viability,DNA fragmentation and MTT assay were carried out.Results:Methanolic extract attenuated percentage increased in the cell survival lime when compared to control group.However,the effect was less than that of methotrexat.Methotrexat and the extracts reversed the tumor-induced alterations in UNA fragmentation and MTT assay.Conclusions:The present study suggests that the methanol extract of Cyathula prostrata has significant anticancer activity and that is comparable to that of methotrexate. Objective: To evaluate the anticancer activity of the methanolic extract of Cyathula prostrata in Ehrlich ascites carcinoma (EAC) -bearing mice with methotrexate as positive control in the advanced stage of tumorigenesis. Methods: EAC was induced in swiss albino mice by injecting 10-6 cells / mL of tumor cell suspension intraperitoneal. methanolic extract of Cyathula prostrata effect on the tumor cell viability, DNA fragmentation and MTT assay were carried out. Results: Methanolic extract attenuated percentage increased in the cell survival lime when compared to control group. Still, the effect was less than that of methotrexat. the Methotrexat. the exploded by the tumor-induced alterations in UNA fragmentation and MTT assay. Conclusions: The present study suggests that the methanol extract of Cyathula prostrata has significant anticancer activity and that is comparable to that of methotrexate