根据补体经典途径激活时，可通过“旁立损伤”机制使邻近正常细胞溶解破坏的原理，该种溶血空斑试验不仅可以用来定量检测分泌ＩｇＭ的抗体形成细胞，也可直接用来定量检测分泌ＩｇＧ的抗体形成细胞。与传统溶血空斑试验相比，其最大的优点是可用来检测机体对任何一种可溶性抗原的特异性体液免疫应答能力，从而扩展了应用范围。 When activated by the classical complement pathway, the principle of adjacent normal cell lysis can be induced by the “bypass injury” mechanism that can be used not only to quantify IgM-producing antibody-producing cells, but also directly for quantitative detection IgG-secreting antibody-forming cells. Compared with the traditional hemolytic plaque test, its biggest advantage is that it can be used to detect the body’s ability to humoral immune response to any one kind of soluble antigen, thus expanding the scope of application.