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目的:观察冻贮细管、开放型拉细冻贮细管和闭合型拉细冻贮细管三种冻贮细管对玻璃化冻融胚胎形态和功能的影响。方法:将人三原核受精卵发育形成的200个6-10-细胞胚胎随机分为四组:对照组20个、冻贮细管组(straw,S)、开放型拉细冻贮细管组(openpulledstraw,OPS)和闭合型拉细冻贮细管组(closepulledstraw,CPS)各60个。比较超速玻璃化冷冻胚胎复苏后存活率;并测定复苏后部分存活胚胎线粒体跨膜电位(△Ψm)。结果:CPS超速玻璃化冷冻胚胎存活率最高,与OPS和S组相比,差异具有显著性(P<0.05);后两者间无差异。CPS与OPS组△Ψm均高于S组(P<0.05);前两者间无明显差异(P>0.05);但实验组△Ψm均低于对照组,差异有统计学意义(P<0.05)。结论:CPS对玻璃化冻融胚胎的形态和功能影响最小;OPS组虽未获得比S组更高的形态存活率,但减轻了对胚胎的功能损伤。
OBJECTIVE: To observe the effects of three kinds of frozen stubble tubes, frozen frozen tube, open slender frozen tube and closed slender frozen tube on morphology and function of vitrified embryos. Methods: 200 6-10-cell embryos formed from the development of human pronuclear fertilized eggs were randomly divided into four groups: control group (20), straw, S group, (openpulled straw, OPS) and closepulled straw (closedpulled straw, CPS) 60 each. To compare the survival rate of resuscitative vitrified embryos after resuscitation; and to determine the mitochondrial transmembrane potential (△ Ψm) of some surviving embryos after resuscitation. Results: The survival rate of CPS over-frozen vitrified embryos was the highest, compared with OPS and S groups, the difference was significant (P <0.05). There was no difference between the two groups. The △ Ψm in both CPS and OPS groups were significantly higher than those in S group (P <0.05). There was no significant difference between the two groups (P> 0.05), but △ Ψm in experimental group was lower than that in control group ). CONCLUSION: CPS has the least effect on the morphology and function of vitrified embryos. Although OPS group does not have higher morphologic survival rate than S group, it can reduce functional impairment of embryos.