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目的通过脐血粒单系造血祖细胞（ＣＦＵ－ＧＭ）培养，探讨其临床应用前景与价值。方法琼脂半固体培养法，比较脐血、骨髓、外周血ＣＦＵ－ＧＭ，比较脐血４℃保存２，４，６天后，ＣＦＵ－ＧＭ回收率。结果脐血ＣＦＵ－ＧＭ的平均产率接近骨髓，明显高于成人外周血。脐血４℃保存，ＣＦＵ－ＧＭ回收率２天为８５．５％，４天为３５．１％，６天为１０．１％。结论脐血中富含造血干／祖细胞，为造血细胞移植提供新的来源。脐血输注应尽量采用新鲜脐血，采血后２４ｈ内输注，也可７２ｈ内输注。
Objective To investigate the clinical application and prospect of cord blood mononuclear hematopoietic progenitor cells (CFU-GM). Methods The agar semi-solid culture method was used to compare the CFU-GM of umbilical cord blood, bone marrow and peripheral blood. The recovery of CFU-GM after 2, 4 and 6 days of cord blood preservation at 4 ℃ was compared. Results The average yield of cord blood CFU-GM was close to that of bone marrow, which was significantly higher than that of adult peripheral blood. Umbilical cord blood was stored at 4 ° C. The recovery rate of CFU-GM was 85.5% in 2 days, 35.1% in 4 days and 10.1% in 6 days. Conclusion Cord blood is rich in hematopoietic stem / progenitor cells, providing a new source for hematopoietic cell transplantation. Umbilical cord blood transfusion should try to use fresh cord blood, blood within 24h after infusion, but also within 72h infusion.