论文部分内容阅读
目的:制定胆炎颗粒质量标准。方法:采用薄层色谱(TLC)法鉴别甘草、大黄、茵陈,高效液相色谱法测定橙皮苷、芍药苷的含量,色谱柱:大连依利特HypersilODS2(150 mm×4.6 mm,5μm),流动相分别为乙腈-0.5%醋酸(17:83)、乙腈-0.1%磷酸溶液(15:85);流速:1.0 ml·min~(-1);检测波长:283 nm、232 nm;柱温:30℃。结果:TLC色谱能明显检出甘草、大黄、茵陈,橙皮苷、芍药苷分别在0.086～0.259μg(r=0.999 7)、0.276～0.829μg(r=0.999 8)范围内呈现良好线性关系,橙皮苷的平均回收率为97.69%,RSD=0.92%(n=6);芍药苷的平均回收率为97.95%,RSD=0.56%(n=6)。结论:本方法简便,准确,重复性好,可用于本品质量控制。
Objective: To establish the quality standards of Chiyan granules. Methods: The content of hesperidin and paeoniflorin in licorice root, rhubarb and capillaris were determined by thin layer chromatography (TLC), and the column was Dalian Hypersil ODS2 (150 mm × 4.6 mm, 5 μm) The mobile phase was acetonitrile-0.5% acetic acid (17:83) and acetonitrile-0.1% phosphoric acid solution (15:85) respectively. The flow rate was 1.0 ml · min -1 and the detection wavelength was 283 nm and 232 nm. : 30 ° C. Results: TLC chromatogram showed that there was a good linear relationship between licorice, rhubarb, capillaris, hesperidin and paeoniflorin in the range of 0.086-0.259μg (r = 0.999 7) and 0.276-0.829μg (r = 0.999 8) The average recovery of hesperidin was 97.69%, RSD was 0.92% (n = 6). The average recovery of paeoniflorin was 97.95% with RSD = 0.56% (n = 6). Conclusion: The method is simple, accurate, reproducible, and can be used for quality control of this product.