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目的本实验是观察大鼠急性胰腺炎后经胃肠道给予庆大霉素或谷氨酰胺对肠道细菌易位的影响。方法结扎封闭群 Wistar 大鼠的胆管,制成急性胰腺炎模型。分假手术组、急性胰腺炎组、庆大霉素组和谷氨酰胺组4组,各组又分5个亚组,分别于术后24、48、72、96和144小时处死动物。无菌下取回盲部淋巴结、胰腺、脾、肝、门静脉血和盲肠内容做细菌培养、计数。胰腺和小肠组织做病理检查。结果表明大鼠急性胰腺炎发生后,肠系膜淋巴结细菌数和阳性率明显增高,与门静脉血比较有显著差异。庆大霉索组盲肠内容和肠系膜淋巴结的大肠杆菌数明显减少,革兰氏阳性菌明显增多。谷氨酰胺组盲肠内容和肠系膜淋巴结的细菌数均显著减少。结论大鼠急性胰腺炎发生后早期肠道细菌易位主要经肠系膜淋巴途径。经胃肠道给予谷氨酰胺可防止肠道内细菌易位,减少胰腺感染的发生。
Objective This experiment was to observe the effects of gentamicin or glutamine on intestinal bacterial translocation after acute pancreatitis in rats. Methods Acute pancreatitis model was established by ligating the bile duct of Wistar rats. The rats in sham operation group, acute pancreatitis group, gentamicin group and glutamine group were divided into 5 subgroups. Animals were killed at 24, 48, 72, 96 and 144 hours after operation. Aseptic removal of blind lymph nodes, pancreas, spleen, liver, portal vein blood and cecal contents of bacterial culture, counting. Pancreas and small intestine tissue pathology. The results showed that after the occurrence of acute pancreatitis in rats, the number of mesenteric lymph nodes and the positive rate was significantly increased, compared with the portal vein blood were significantly different. The amount of E. coli in the caecum contents and mesenteric lymph nodes in the gentamicin group was significantly reduced, and the gram-positive bacteria were significantly increased. The amount of cecal contents and mesenteric lymph nodes in the glutamine group were significantly reduced. Conclusion Early intestinal bacterial translocation mainly occurs in the mesenteric lymph node after acute pancreatitis in rats. Glutamine administered via the gastrointestinal tract prevents bacterial translocation in the intestine and reduces the incidence of pancreatic infections.