论文部分内容阅读
目的通过对大连市一起“蜱咬”患者流行病学调查及病原体检测,探讨新布尼亚病毒(Severe fever with throm-bocytopenia syndrome bunyavirus,SFTSV)在辽宁省南部沿海地区的流行范围。方法采用ELISA方法对“蜱咬”患者急性期血清样本及同居住地的26份非患者血清样本进行SFTSV的IgM、IgG抗体检测;采用实时荧光定量RT-PCR方法检测“蜱咬”患者血液样本SFTSV核酸;采用VERO-E6细胞对“蜱咬”患者血液样本进行病毒分离并鉴定。结果 “蜱咬”患者急性期血清样本SFTSV的IgM抗体阳性、IgG抗体阴性;26份非患者血清样本中4份SFTSV的IgG抗体阳性;“蜱咬”患者血液样本SFTSV核酸阳性;从“蜱咬”患者血液样本和体表叮咬的蜱虫中各分离出1株病毒,经SFTSV的M片段全基因测序引物测序鉴定为SFTSV。结论 “蜱咬”患者血液分离株与蜱虫分离株均为SFTSV,同源性达99.7%,该病毒是导致患者发病的重要原因。本次分离到的2株病毒与我省东部(丹东、抚顺),北部(铁岭)山区分离株遗传距离相对较远。SFTSV在正常人群中存在隐性感染者。
Objective To investigate the prevalence of Severe fever with throm-bocytopenia syndrome bunyavirus (SFTSV) in the southern coastal areas of Liaoning Province through epidemiological investigation and pathogen detection of “tick bite” in Dalian. Methods Serum samples from acute phase and “non-patient” of 26 ticks were detected by ELISA for the detection of IgM and IgG antibodies to SFTSV. The real-time fluorescent quantitative RT-PCR method was used to detect the “tick bite” SFTSV nucleic acid for patient blood samples; Vero-E6 cells were virus-isolated and identified for blood samples from “tick bite” patients. RESULTS: Serum samples of “ticks” were positive for serum IgM antibodies to SFTSV and negative for IgG antibodies; IgG antibodies to 4 SFTSV were positive for serum from 26 non-patient serum samples; SFTSV nucleic acid was positive for blood samples from “ticks” One strain of virus was isolated from the blood samples of tick ticks and tick ticks of the body surface and identified as SFTSV by sequencing of the full-length M-segment gene of SFTSV. Conclusion The results showed that SFTSV was isolated from the tick tick bite and its homology was 99.7%. This virus is an important cause of the disease. The two isolates isolated from this area are relatively far away from the mountainous isolates in the east (Dandong, Fushun) and northern (Tieling) mountains. SFTSV There are latent infections in the normal population.