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我们在GM—CFU传统体外琼脂培养(简称全量法)基础上,参照葛忠良等建立的GM—CFC体外微量法(筒称微量法),对“正常人”骨髓及3.5～5个月胎龄的胎肝细胞悬液各9例进行了GM—CFU体外微量琼脂培养。“正常人”骨髓取自本院胸科手术中切除的肋骨,半小时内将骨髓细胞挤压在RPMI1640液,用吸管打散,过7号及41/2号针头后以2000r/min离心10min。弃上清,加适量RPMI1640液打散,淋巴细胞分层液(比重1.077)4ml的离心管内加上骨髓细胞分层以1700r/min离心25min.
On the basis of GM-CFU traditional in vitro agar culture (referred to as the full-scale method), with reference to the GM-CFC in vitro method developed by Ge Zhongliang et al., The “normal” bone marrow and 3.5 to 5 months gestational age Of fetal liver cell suspension in 9 cases of GM-CFU in vitro micro agar culture. “Normal” bone marrow taken from our hospital thoracotomy resection of the ribs, bone marrow cells within half an hour squeeze in RPMI1640 solution, using a pipette to break up, after 7 and 41/2 needle to 2000r / min centrifugal 10min . Discard the supernatant, add appropriate amount of RPMI1640 liquid to disperse, lymphocyte stratified liquid (specific gravity 1.077) 4ml centrifuge tube with bone marrow cells layered to 1700r / min centrifugation 25min.