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目的:探讨CCR5Δ32等位基因在贵州世居少数民族(彝、瑶)与汉族人群的分布,并分析CCR5位点突变与HBV的关系.方法:采用PCR技术分别扩增贵州92份黔西彝族、101份威宁彝族、138份荔波瑶族以及165份毕节汉族CCR5编码区片段,通过琼脂糖凝胶电泳对CCR5Δ32多态性进行分析,最后抽取样本并进行DNA测序验证.结果:经过PCR特异性扩增,琼脂糖凝胶电泳后,496份样本均未发现CCR5Δ32突变型和杂合子.DNA测序验证所有样本CCR5基因未发生32碱基缺失的突变.结论:CCR5Δ32的分布有较明显的地域和种族差异,该位点与HBV感染的相关性有待于进一步深入研究.
Objective: To investigate the distribution of CCR5Δ32 alleles in Guizhou ethnic minority population (Yi, Yao) and Han population and to analyze the relationship between CCR5 locus mutation and HBV.Methods: PCR was used to amplify 92 Yi ethnic groups in Guizhou province, 101 copies of Weining Yi, 138 Libo Yao and 165 Bijie Han CCR5 coding region fragments were analyzed by agarose gel electrophoresis CCR5Δ32 polymorphism, the final sample was extracted and DNA sequencing results.Results: After PCR-specific After amplification and agarose gel electrophoresis, no CCR5Δ32 mutant and heterozygote were found in 496 samples.DNA sequencing confirmed that no CCR5 gene mutation occurred in all the samples.Conclusion: The distribution of CCR5Δ32 has obvious geographical and Racial differences, the relationship between HBV infection and the site needs further study.