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微卫星标记因其具有等位基因丰富、检测技术简单等优点,成为近几年迅速发展的一种分子标记技术。微卫星的一个具有潜在价值的特征,就是从一个物种产生的引物可以应用于相关的分类群。试验尝试利用合成的2对小麦的SSR引物(Xgwm23D、Xgwm323A),通过梯度PCR对克氏针茅和冰草的DNA进行PCR扩增,结果一对引物(Xgwm323A)在两者中均有扩增,初步摸清了小麦微卫星引物(Xgwm323A)在克氏针茅和冰草中PCR反应条件,为进一步研究奠定了基础。
Because of its advantages of allele richness and simple detection technique, microsatellite markers have become a kind of molecular marker technology that has developed rapidly in recent years. A potentially valuable feature of microsatellites is that primers derived from one species can be applied to the relevant taxon. In the experiment, two pairs of wheat SSR primers (Xgwm23D, Xgwm323A) were synthesized and used for PCR amplification of DNA of Stipa krylovii and Agropyron sp. By PCR. As a result, a pair of primers (Xgwm323A) were amplified in both , Preliminarily found out the PCR reaction conditions of wheat microsatellite DNA primer (Xgwm323A) in Stipa krylovii and Agropyron chinensis, which lays the foundation for further research.