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目的 :比较软骨细胞、骨髓基质细胞及成纤维细胞对全层关节软骨缺损的修复作用。材料和方法 :取幼兔的软骨细胞、骨髓基质细胞及成纤维细胞 ,共 3种有生成软骨潜力的细胞进行体外分离培养 ;以聚乳酸 (PLA)为载体 ,将培养的原代细胞植入PLA支架上 ,形成细胞 -PLA复合物。于 2 8只成年新西兰大白兔的股骨滑车关节面上造成直径 4 5mm、深 3 0mm的全层关节软骨缺损 ,将 3种细胞 -PLA复合物分别植入关节软骨缺损处。植入细胞 -PLA复合物为实验组 ,单纯植入PLA支架为对照组。术后 6周、12周观察缺损修复情况及新生组织类型。结果 :软骨细胞移植组为软骨样组织修复 ,分界明显 ,甲苯胺兰及Ⅱ型胶原染色阳性 ;软骨下骨部分重建 ;细胞排列紊乱。骨髓基质细胞移植组为软骨样组织修复 ,分界不明显 ,甲苯胺兰及Ⅱ型胶原染色阳性 ;软骨下骨重建良好 ,软骨下潮线恢复 ;细胞排列趋于正常。成纤维细胞移植组为纤维组织修复 ,甲苯胺兰及Ⅱ型胶原染色阴性 ;软骨下潮线消失。对照组为纤维组织修复。结论 :软骨细胞、骨髓基质细胞移植修复软骨缺损明显优于成纤维细胞及对照组。骨髓基质细胞与软骨细胞移植组的修复结果无统计学差异 ,但骨髓基质细胞修复组织的细胞排列有序 ,软骨下骨重建良好 ,与周围组织融合密切 ,更接近正?
OBJECTIVE: To compare the repair effect of chondrocytes, bone marrow stromal cells and fibroblasts on the full thickness articular cartilage defects. MATERIALS AND METHODS: Chondrocytes, bone marrow stromal cells and fibroblasts were obtained from rabbits. Three cartilage-producing cells were isolated and cultured in vitro. Polylactide (PLA) PLA scaffold to form a cell-PLA complex. Thirty-five full-thickness articular cartilage defects of 4 mm in diameter and 30 mm in depth were created on the articular surface of the femur of 28 adult New Zealand white rabbits. Three types of cell-PLA complexes were implanted into the articular cartilage defects respectively. The PLA-PLA complex was implanted into the experimental group and the PLA scaffold was implanted into the control group. After 6 weeks, 12 weeks to observe the defect repair and tissue types. RESULTS: Chondrocyte-like tissue was repaired by cartilage-like tissue. The demarcation was obvious. Toluidine blue and type Ⅱ collagen staining were positive. The subchondral bone was partly reconstructed. The cells were disordered. Bone marrow stromal cells transplantation group was cartilage-like tissue repair, the border is not obvious, toluidine blue and collagen type Ⅱ staining; subchondral bone reconstruction well, subchondral tidal line recovery; cell arrangement tends to normal. Fibroblast transplantation group for the repair of fibrous tissue, toluidine blue and collagen type Ⅱ staining; subchondral line disappeared. The control group was fibrous tissue repair. Conclusion: Chondrocytes and bone marrow stromal cells transplantation are superior to fibroblasts and control group in repairing cartilage defects. The results of bone marrow stromal cells and chondrocyte transplantation group showed no statistical difference, but the cells of bone marrow stromal cells were arranged in order, the subchondral bone reconstructed well and closely fused with the surrounding tissues,