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以偏苯三酸酐、4-氯间苯二酚为原料,在ZnCl2催化下反应得到2’,7’-二氯-5(6)-羧基荧光素混合物,并用柱色谱进行异构体分离。对取代基团对其荧光性能影响的研究发现,顶环上引入氯使其最大荧光激发波长和荧光发射波长发生红移,荧光强度有所增加;底环上引入羧基,其Stokes位移和荧光量子产率略有降低,但该活性基团的引入将更有利于对生物体进行检测。由此表明,2’,7’-二氯-5(6)-羧基荧光素有望用于荧光探针。
Using trimellitic anhydride and 4-chlororesorcinol as raw materials, the reaction of 2 ’, 7’-dichloro-5 (6) -carboxyfluorescein under ZnCl 2 catalysis was carried out and the isomers were separated by column chromatography. The study on the influence of substituent group on its fluorescence showed that the maximum fluorescence excitation wavelength and fluorescence emission wavelength were red-shifted by introducing chlorine on the top ring, and the fluorescence intensity increased. The carboxyl groups were introduced into the bottom ring, and the Stokes shift and fluorescence quantum The yield is slightly reduced, but the introduction of the active group will be more conducive to the detection of organisms. This indicates that 2 ’, 7’-dichloro-5 (6) -carboxyfluorescein is expected to be used as a fluorescent probe.