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目的:探讨子宫内膜单胺氧化酶A(MAO-A)在评估子宫内膜容受性中的作用。方法:应用实时荧光定量PCR和免疫组织化学技术,检测3次IVF-ET失败患者(A组,n=39)、2次IVF-ET失败,第3次获得临床妊娠患者(B组,n=8)、首次IVF-ET获得临床妊娠患者(C组,n=21)在子宫内膜种植窗口期子宫内膜MAO-A mRNA的表达和MAO-A蛋白的表达及定位,同时扫描电子显微镜观察子宫内膜的胞饮突数量及发育情况,探讨子宫内膜MAO-A在评估子宫内膜容受性中的作用。结果:反复IVF-ET失败患者在子宫内膜种植窗口期子宫内膜MAO-A的表达低于首次IVF-ET获得临床妊娠的患者(P<0.05)。结论:子宫内膜MAO-A可以作为子宫内膜容受性的预测指标。
Objective: To investigate the role of endometrial monoamine oxidase A (MAO-A) in the assessment of endometrial receptivity. Methods: Three failures of IVF-ET (group A, n = 39), two failures of IVF-ET and the third of patients with clinical pregnancy were detected by real-time fluorescence quantitative PCR and immunohistochemistry (group B, n = 8). The expression of MAO-A mRNA and the expression of MAO-A mRNA in endometrium of endometrial implantation window group (C group, n = 21) were obtained from the first IVF-ET. Scanning electron microscopy Endometrial number of esophageal processes and development, to explore the role of endometrial MAO-A in the assessment of endometrial receptivity. Results: The expression of MAO-A in endometrium was lower in IVF-ET failure patients than in IVF-ET patients with clinical pregnancy (P <0.05). Conclusion: Endometrial MAO-A can be used as a predictor of endometrial receptivity.