论文部分内容阅读
目的:利用实时荧光定量PCR检测赖氨酰氧化酶样-4(Lysyloxidase-like 4,LOXL4)基因在Hela、ME180、HCC94人宫颈癌细胞中的表达情况。方法:提取3株体外培养宫颈癌细胞株的总RNA,逆转录为cDNA,实时荧光定量PCR扩增,结合内参基因GAPDH及标准曲线比较LOXL4 mRNA在Hela、ME180和HCC94 3株体外培养的人宫颈癌细胞中的表达。结果:LOXL4基因在3株人宫颈癌细胞中均有表达,但表达水平存在统计学差异,以Hela的表达为参照(Ratio=LOXL4/GAPDH=1.00),ME180的表达水平为6.80E-3,HCC94细胞株表达丰度最低为5.62E-4。结论:建立了LOXL4基因在宫颈癌细胞表达的检测平台,为宫颈癌早期诊断及预测预后提供可借鉴的观察指标。
Objective: To detect the expression of Lysyloxidase-like 4 (LOXL4) gene in Hela, ME180 and HCC94 human cervical carcinoma cells by real-time fluorescence quantitative PCR. Methods: The total RNA was extracted from 3 cervical cancer cell lines in vitro and reverse transcribed into cDNA. Real-time PCR was used to amplify the cDNA of LOXL4 mRNA in human cervical cancer cell line Hela, ME180 and HCC94 in combination with the reference gene GAPDH and standard curve Expression in cancer cells. Results: The LOXL4 gene was expressed in all three human cervical cancer cells, but the expression level was statistically different. The expression level of ME180 was 6.80E-3 with reference to Hela expression (Ratio = LOXL4 / GAPDH = 1.00) The lowest expression level of HCC94 cell line was 5.62E-4. Conclusion: The detection platform of LOXL4 gene expression in cervical cancer cells was established, which could provide reference for the early diagnosis and prognosis of cervical cancer.