本文报道采用高效表达载体PBS(tac启动子)在大肠杆菌(E·coli)中表达乙型肝炎病毒核心抗原(HBcAg)的方法.重组质粒在异丙基一硫代半乳糖苷(IPTG)的诱导下,其表达效率提高近1000倍;经溶菌酶—SDS破壁、DNase处理后,离心上清液中含有大量HBcAg.通过对IPTG诱导浓度、诱导时间及细菌起始诱导浓度等因素的研究.我们找到一系列高效表达HB-cAg的条件,表达滴度高达1:25600,达到国内同类先进水平 In this paper, a method for the expression of hepatitis B virus core antigen (HBcAg) in E. coli using the highly efficient expression vector PBS (tac promoter) was reported. The recombinant plasmid was expressed in isopropylthiogalactoside (IPTG) Induced by E.coli, its expression efficiency increased by nearly 1000-fold.After treated with lysozyme-SDS and DNase, a large amount of HBcAg was contained in the supernatant.The inducing concentration, inducing time and initial concentration of bacterium induced by IPTG We found a series of efficient expression of HB-cAg conditions, the expression of titers up to 1: 25600, reached the advanced level of similar domestic